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AGRP ELISA Kit

Rockland koa0862 AGRP Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Rockland
Catalog No. ABIN6720335
Supplier Product No.: koa0862
  • Target See all AGRP ELISA Kits
    AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
    Reactivity
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8 pg/mL - 500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0862
    Supplier
    Rockland
    Purpose
    Human AGRP - agouti-related Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human AGRP. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 4 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product AGRP ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human AGRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human AGRP standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: sf21, Immunogen sequence: A21-T132

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all AGRP ELISA Kits
    AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
    Alternative Name
    AGRP (AGRP Products)
    Background

    Synonyms: Agrp, AGRT, ART, ASIP2

    Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

    Gene ID
    181
    NCBI Accession
    NP_001129
    UniProt
    O00253
    Pathways
    Feeding Behaviour, Photoperiodism
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