AGRP ELISA Kit

Catalog No. ABIN6720335

Supplier Product No.: koa0862

Product Details AGRP ELISA Kit

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Supplier Product No.: koa0862

Supplier: Rockland

Purpose: Human AGRP - agouti-related Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human AGRP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 4 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human AGRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human AGRP standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: A21-T132

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Alternative Name: AGRP (AGRP Products)

Background:

Synonyms: Agrp, AGRT, ART, ASIP2

Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Gene ID: 181

NCBI Accession: NP_001129

UniProt: O00253

Pathways: Feeding Behaviour, Photoperiodism