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TIMP4 ELISA Kit

Rockland koa0316 TIMP4 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720333
Supplier Product No.: koa0316
  • Target See all TIMP4 ELISA Kits
    TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
    Reactivity
    • 14
    • 13
    • 8
    • 8
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0316
    Supplier
    Rockland
    Purpose
    Human TIMP-4 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product TIMP4 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or human milk to each empty well. We recommend that each human TIMP-4 standard solution and each sample is measured in duplicate.
    Comment

    Standard: Expression system for standard: sf21, Immunogen sequence: C30-P224

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all TIMP4 ELISA Kits
    TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
    Alternative Name
    TIMP-4 (TIMP4 Products)
    Background

    Synonyms: Metalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4

    Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

    Gene ID
    7079
    NCBI Accession
    NP_003247
    UniProt
    Q99727
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