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DPP4 ELISA Kit

Rockland koa0401 DPP4 Reactivity: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Rockland
Catalog No. ABIN6720324
Supplier Product No.: koa0401
  • Target See all DPP4 ELISA Kits
    DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
    Reactivity
    • 11
    • 11
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0401
    Supplier
    Rockland
    Purpose
    Human CD26 - DPP4 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human CD26. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product DPP4 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CD26 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) saliva or urine to each empty well. It is recommended that each human CD26 standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: D34-P766

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all DPP4 ELISA Kits
    DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
    Alternative Name
    CD26/DPP4 (DPP4 Products)
    Background

    Synonyms: ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4, DPP 4, DPP IV, DPP4, DPP4_HUMAN, DPPIV, T-cell activation antigen CD26, TP103

    Background: Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

    Gene ID
    1803
    NCBI Accession
    NP_001926
    UniProt
    P27487
    Pathways
    Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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