CRISP3 ELISA Kit

Catalog No. ABIN6720320

Supplier Product No.: koa0760

Product Details CRISP3 ELISA Kit

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Supplier Product No.: koa0760

Supplier: Rockland

Purpose: Human CRISP3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CRISP3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRISP3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or tissue homogenates to each empty well. We recommend that each human CRISP3 standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: N21-Y245

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CRISP3

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Alternative Name: CRISP3 (CRISP3 Products)

Background:

Synonyms: Aeg 2, Aeg2, CRIS3_HUMAN, CRISP 3, CRISP-3, Crisp3, CRS 3, CRS3, Cysteine rich secretory protein 3, Cysteine-rich secretory protein 3, dJ442L6.3, MGC126588, OTTHUMP00000016590, OTTHUMP00000039911, SGP 28, SGP28, SGP28 protein, Specific granule protein (28 kDa), Specific granule protein of 28 kDa

Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Gene ID: 10321

NCBI Accession: NP_001177915

UniProt: P54108