Adipsin ELISA Kit
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- Target See all Adipsin (CFD) ELISA Kits
- Adipsin (CFD) (Complement Factor D (CFD))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 312 pg/mL - 20000 pg/mL
- Minimum Detection Limit
- 312 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0696
- Supplier
- Rockland
- Purpose
- Human CFD Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human CFD . There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CFD ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CFD standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human CFD standard solution and each sample is measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: I26-A253
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all Adipsin (CFD) ELISA Kits
- Adipsin (CFD) (Complement Factor D (CFD))
- Alternative Name
- CFD (CFD Products)
- Background
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Synonyms: Adipsin, ADN, C3 convertase activator, CFAD_HUMAN, CFD, Complement factor D, Complement factor D preproprotein, D component of complement, DF, PFD, Properdin factor D
Background: Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
- Gene ID
- 1675
- NCBI Accession
- NP_001304264
- UniProt
- P00746
- Pathways
- Complement System
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