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Adipsin ELISA Kit

Rockland koa0696 CFD Reactivity: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
Rockland
Catalog No. ABIN6720317
Supplier Product No.: koa0696
  • Target See all Adipsin (CFD) ELISA Kits
    Adipsin (CFD) (Complement Factor D (CFD))
    Reactivity
    • 8
    • 4
    • 3
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0696
    Supplier
    Rockland
    Purpose
    Human CFD Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human CFD . There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CFD ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CFD standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human CFD standard solution and each sample is measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: I26-A253

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all Adipsin (CFD) ELISA Kits
    Adipsin (CFD) (Complement Factor D (CFD))
    Alternative Name
    CFD (CFD Products)
    Background

    Synonyms: Adipsin, ADN, C3 convertase activator, CFAD_HUMAN, CFD, Complement factor D, Complement factor D preproprotein, D component of complement, DF, PFD, Properdin factor D

    Background: Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

    Gene ID
    1675
    NCBI Accession
    NP_001304264
    UniProt
    P00746
    Pathways
    Complement System
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