SERPINC1 ELISA Kit

Catalog No. ABIN6720315

Product Details SERPINC1 ELISA Kit

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: ANT3_HUMAN, Antithrombin, Antithrombin III, Antithrombin-III, AntithrombinIII, AT 3, AT III, AT3, AT3D, ATIII, Heparin cofactor I, MGC22579, Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1, Serine cysteine proteinase inhibitor clade C member 1, Serine proteinase inhibitor clade C member 1, Serpin C1, Serpin peptidase inhibitor clade C (antithrombin) member 1, SERPINC1, THPH7

Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).

Gene Name: SERPINC1

Production: Natural and recombinant human Serpin C1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: H33-K464

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for SERPINC1

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: Serpin C1 - Antithrombin-III (SERPINC1 Products)

Synonyms: AT3 ELISA Kit, AT3D ELISA Kit, ATIII ELISA Kit, THPH7 ELISA Kit, SERPINC1 ELISA Kit, DKFZp470C1733 ELISA Kit, AI114908 ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, ANTITHROMBIN, AT-III ELISA Kit, AT-III ELISA Kit, serpin family C member 1 ELISA Kit, antithrombin-III ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, SERPINC1 ELISA Kit, CpipJ_CPIJ000472 ELISA Kit, CpipJ_CPIJ013111 ELISA Kit, Serpinc1 ELISA Kit

Gene ID: 462

NCBI Accession: NP_000479

UniProt: P01008