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BIRC7 ELISA Kit

Rockland koa0812 BIRC7 Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate
Rockland
Catalog No. ABIN6720313
Supplier Product No.: koa0812
  • Target See all BIRC7 ELISA Kits
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Reactivity
    • 8
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0812
    Supplier
    Rockland
    Purpose
    Human Livin Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human Livin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 20 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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    Discover our top product BIRC7 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Livin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, cell lysates or tissue homogenates to each empty well. It is recommended that each human Livin standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: M1-S298

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all BIRC7 ELISA Kits
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Alternative Name
    Livin (BIRC7 Products)
    Background

    Synonyms: Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, Baculoviral IAP repeat-containing protein 7, BIRC 7, BIRC7, BIRC7_HUMAN, KIAP, Kidney inhibitor of apoptosis protein, LIVIN, Livin inhibitor of apoptosis, Melanoma inhibitor of apoptosis protein, ML IAP, ML-IAP, MLIAP, RING finger protein 50, RNF 50, RNF50

    Background: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.

    Gene ID
    79444
    NCBI Accession
    NP_071444
    UniProt
    Q96CA5
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