TXN ELISA Kit

Catalog No. ABIN6720303

Product Details TXN ELISA Kit

Target: TXN (Thioredoxin (TXN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: ADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein

Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

Gene Name: TXN

Production: Natural and recombinant human Thioredoxin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: V2-V105

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TXN

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin (TXN Products)

Synonyms: TRDX ELISA Kit, TRX ELISA Kit, TRX1 ELISA Kit, ADF ELISA Kit, AW550880 ELISA Kit, Trx1 ELISA Kit, Txn ELISA Kit, trx ELISA Kit, TXN ELISA Kit, SCM1.18 ELISA Kit, trxA3 ELISA Kit, thioredoxin ELISA Kit, TR-1 ELISA Kit, Trx-1 ELISA Kit, thioredoxin ELISA Kit, thioredoxin 1 ELISA Kit, thioredoxin L homeolog ELISA Kit, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) ELISA Kit, thioredoxin pseudogene ELISA Kit, cytosolic thioredoxin Trx1 ELISA Kit, TXN ELISA Kit, Txn1 ELISA Kit, txn.L ELISA Kit, txn ELISA Kit, trx ELISA Kit, SCO0885 ELISA Kit, PHG25ORF099c ELISA Kit, LOC100349046 ELISA Kit, Txn ELISA Kit, trx1 ELISA Kit

Gene ID: 7295

NCBI Accession: NP_001231867

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis