CCL24 ELISA Kit

Catalog No. ABIN6720293

Supplier Product No.: koa0161

Product Details CCL24 ELISA Kit

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Supplier Product No.: koa0161

Supplier: Rockland

Purpose: Human CCL24 - Eotaxin-2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CCL24. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CCL24 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL24 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: V27-C119

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: CCL24/Eotaxin-2 (CCL24 Products)

Background:

Synonyms: C C motif chemokine 24, CCL24, Chemokine (C C motif) ligand 24, Chemokine CC Motif Ligand 2, CK beta 6, Ckb 6, Ckb6, Eosinophil chemotactic protein 2, Eotaxin 2 like protein, MPIF 2, MPIF2, Myeloid progenitor inhibitory factor 2, OTTHUMP00000210225, SCYA24, Small inducible cytokine A24, Small inducible cytokine subfamily A (Cys Cys) member 24

Background: Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Gene ID: 6369

NCBI Accession: NP_002982

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization