Relaxin 2 ELISA Kit

Catalog No. ABIN6720288

Product Details Relaxin 2 ELISA Kit

Target: Relaxin 2 (RLN2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: H2, Relaxin H2, RelaxinH2, Relaxin-H2, Relaxin 2, Relaxin2, Relaxin-2, RLN 2, RLN2, RLN-2, Relaxin A chain, RLXH2

Background: Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The active form of the protein encoded by this gene consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Relaxin 2(RLN2) is a polypeptide hormone structurally related to insulin and insulin-like growth factors (IGFs). However it does not interact with insulin receptors and has decidedly different biological properties. Relaxin 2 activates LGR 7 and LGR 8 relaxin receptors, that belong to the leucine-rich repeat-containing (LRR), G protein-coupled receptors.

Gene Name: Relaxin 2

Production: Natural and recombinant human Relaxin 2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: Q162-C185(A)&D25-S53(B)

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human Relaxin 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Relaxin 2 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Relaxin 2

Target: Relaxin 2 (RLN2)

Alternative Name: Relaxin 2 (RLN2 Products)

Synonyms: H2 ELISA Kit, H2-RLX ELISA Kit, RLXH2 ELISA Kit, bA12D24.1.1 ELISA Kit, bA12D24.1.2 ELISA Kit, RFLX ELISA Kit, rln2 ELISA Kit, rln3c ELISA Kit, rlx3c ELISA Kit, si:rp71-39b20.5 ELISA Kit, relaxin 2 ELISA Kit, insulin-like 3 (Leydig cell) ELISA Kit, RLN2 ELISA Kit, insl3 ELISA Kit

Gene ID: 6019

NCBI Accession: NP_005050

UniProt: P04090

Pathways: Hormone Activity