CCL19 ELISA Kit

Catalog No. ABIN6720271

Supplier Product No.: koa0265

Product Details CCL19 ELISA Kit

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Supplier Product No.: koa0265

Supplier: Rockland

Purpose: Mouse CCL19 - MIP-3 beta Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CCL19. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL19 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL19 standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence:  G26-S108

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: CCL19/MIP-3 beta (CCL19 Products)

Background:

Synonyms: Beta chemokine exodus 3, Beta-chemokine exodus-3, C C chemokine ligand 19, C-C motif chemokine 19, CC chemokine ligand 19, CCL19_HUMAN, Chemokine ligand 19, Chemokine ligand 19, Chemokine CC Motif Ligand 19, CK beta 11, CK beta-11, CKb11, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine, Exodus 3, Macrophage inflammatory protein 3 beta, MIP 3 beta, MIP-3-beta, MIP-3B, MIP3 beta, MIP3B, SCYA19, Small inducible cytokine A19, Small-inducible cytokine A19

Background: Chemokine(C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Gene ID: 24047

NCBI Accession: NP_036018

UniProt: O70460

Pathways: Positive Regulation of Immune Effector Process