ANGPTL4 ELISA Kit

Catalog No. ABIN6720260

Product Details ANGPTL4 ELISA Kit

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625 pg/mL - 40000 pg/mL

Minimum Detection Limit: 625 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 425O18 1, Angiopoietin like 4, Angiopoietin related protein 4, ANGPT L2 , ANGPT L4, ANGPTL2, ANGPTL4 , Fasting induced adipose factor , FIAF, Fibrinogen/angiopoietin related protein, Hepatic fibrinogen/angiopoietin related protein , HFARP, Major histocompatibility complex region NG27, NL2, Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein , PGAR, pp1158 , PPARG angiopoietin related protein , PSEC0166 , Secreted protein Bk89, Weakly similar to angiopoietin 1

Background: Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Gene Name: ANGPTL4

Production: Natural and recombinant human ANGPTL4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L165-S406

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ANGPTL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for ANGPTL4

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Alternative Name: ANGPTL4 (ANGPTL4 Products)

Synonyms: ANGPTL2 ELISA Kit, ARP4 ELISA Kit, FIAF ELISA Kit, HFARP ELISA Kit, NL2 ELISA Kit, PGAR ELISA Kit, pp1158 ELISA Kit, Arp4 ELISA Kit, Bk89 ELISA Kit, Fiaf ELISA Kit, Hfarp ELISA Kit, Ng27 ELISA Kit, Pgar ELISA Kit, Pgarg ELISA Kit, Pp1158 ELISA Kit, ANGPTL4 ELISA Kit, fiaf ELISA Kit, im:7144703 ELISA Kit, si:rp71-39b20.7 ELISA Kit, angiopoietin like 4 ELISA Kit, angiopoietin-like 4 ELISA Kit, ANGPTL4 ELISA Kit, Angptl4 ELISA Kit, angptl4 ELISA Kit

Gene ID: 51129

NCBI Accession: NP_001034756

UniProt: Q9BY76

Pathways: Regulation of Lipid Metabolism by PPARalpha