SFTPD ELISA Kit
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- Target See all SFTPD ELISA Kits
- SFTPD (Surfactant Protein D (SFTPD))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 625 pg/mL - 40000 pg/mL
- Minimum Detection Limit
- 625 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0674
- Supplier
- Rockland
- Purpose
- Human SP-D Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human SP-D. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 20 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product SFTPD ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SP-D standard solution and each sample be measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: A21-F375
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all SFTPD ELISA Kits
- SFTPD (Surfactant Protein D (SFTPD))
- Alternative Name
- SP-D (SFTPD Products)
- Background
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Synonyms: COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, SP-D, Surfactant associated protein pulmonary 4, Surfactant pulmonary associated protein D
Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
- Gene ID
- 6441
- NCBI Accession
- NP_003010
- UniProt
- P35247
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