TDGF1 ELISA Kit
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- Target See all TDGF1 ELISA Kits
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 62.5 pg/mL - 4000 pg/mL
- Minimum Detection Limit
- 62.5 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0848
- Supplier
- Rockland
- Purpose
- Mouse Cripto - TDGF1 Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant mouse Cripto. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: R26-Q150
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all TDGF1 ELISA Kits
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
- Alternative Name
- Cripto/TDGF1 (TDGF1 Products)
- Background
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Synonyms: CR, CRGF, Cripto 1, Cripto 1 growth factor, cripto, Cripto-1 growth factor, Cripto1, Cripto1 growth factor, Epidermal growth factor like cripto protein CR1, Epidermal growth factor-like cripto protein CR1, TDGF 1, TDGF1, TDGF1_HUMAN, Teratocarcinoma derived growth factor 1, Teratocarcinoma-derived growth factor 1
Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
- Gene ID
- 21667
- NCBI Accession
- NP_033545
- UniProt
- P51865
- Pathways
- EGFR Signaling Pathway
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