DPP4 ELISA Kit

Catalog No. ABIN6720242

Product Details DPP4 ELISA Kit

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4, DPP 4, DPP IV, DPP4, DPP4_HUMAN, DPPIV, T-cell activation antigen CD26, TP103

Background: Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Gene Name: DPP4

Production: Natural and recombinant mouse CD26. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S29-H760

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD26 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD26 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for DPP4

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Alternative Name: CD26 - DPP4 (DPP4 Products)

Synonyms: ADABP ELISA Kit, ADCP2 ELISA Kit, CD26 ELISA Kit, DPPIV ELISA Kit, TP103 ELISA Kit, MGC81966 ELISA Kit, MOP9.8 ELISA Kit, MOP9_8 ELISA Kit, DPP4 ELISA Kit, si:ch73-2d23.3 ELISA Kit, Cd26 ELISA Kit, Dpp-4 ELISA Kit, THAM ELISA Kit, dipeptidyl peptidase 4 ELISA Kit, dipeptidyl-peptidase 4 S homeolog ELISA Kit, prolyl oligopeptidase family protein ELISA Kit, dipeptidyl-peptidase 4 ELISA Kit, dipeptidylpeptidase 4 ELISA Kit, DPP4 ELISA Kit, dpp4.S ELISA Kit, AT5G24260 ELISA Kit, dpp4 ELISA Kit, Dpp4 ELISA Kit

Gene ID: 13482

NCBI Accession: NP_001153015

UniProt: P28843

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity