NGFR ELISA Kit

Catalog No. ABIN6720237

Product Details NGFR ELISA Kit

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD271, CD271 antigen, Gp80 LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Nerve growth factor receptor, Nerve growth factor receptor TNFR superfamily member 16, NGF receptor, NGFR, p75 ICD, p75 ICD, p75 Neurotrophin receptor, p75NTR, TNFR Superfamily Member 16, TNFRSF16, TNR16_HUMAN, Tumor necrosis factor receptor superfamily member 16

Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.

Gene Name: NGFR

Production: Natural and recombinant human TNFRSF16. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: K29-N250

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: TNFRSF16 - NGFR (NGFR Products)

Synonyms: ngfr ELISA Kit, wu:fb38c02 ELISA Kit, wu:fc10h01 ELISA Kit, CD271 ELISA Kit, Gp80-LNGFR ELISA Kit, TNFRSF16 ELISA Kit, p75(NTR) ELISA Kit, p75NTR ELISA Kit, LNGFR ELISA Kit, RNNGFRR ELISA Kit, Tnfrsf16 ELISA Kit, p75 ELISA Kit, NRH1b ELISA Kit, fullback ELISA Kit, nrh1 ELISA Kit, p75ntr ELISA Kit, tnfrsf16 ELISA Kit, p75NGFR ELISA Kit, nerve growth factor receptor ELISA Kit, neurotrophin receptor associated death domain ELISA Kit, nerve growth factor receptor S homeolog ELISA Kit, nerve growth factor receptor (TNFR superfamily, member 16) ELISA Kit, NGFR ELISA Kit, nradd ELISA Kit, Ngfr ELISA Kit, ngfr.S ELISA Kit

Gene ID: 4804

NCBI Accession: NP_002498

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding