IL29 ELISA Kit

Catalog No. ABIN6720230

Product Details IL29 ELISA Kit

Target: IL29 (Interleukin 29 (IL29))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Cytokine ZCYTO21, IFN lambda 1, IFN-lambda-1, IFNL1, IL 29, IL-29, IL29, IL29_HUMAN, Interferon lambda 1, Interferon lambda-1, Interferon, lambda 1, Interleukin 29, Interleukin-29

Background: Interferon lambda-1, also called Interleukin-29(IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.

Gene Name: IFNL1

Production: Natural and recombinant human IL-29. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G20-T200

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-29 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-29 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL29

Target: IL29 (Interleukin 29 (IL29))

Alternative Name: IL-29 (IL29 Products)

Synonyms: IL-29 ELISA Kit, IL29 ELISA Kit, interferon lambda 1 ELISA Kit, interferon lambda-1 ELISA Kit, interleukin 29 (interferon, lambda 1) ELISA Kit, IFNL1 ELISA Kit, LOC612539 ELISA Kit, IL29 ELISA Kit

Gene ID: 282618

NCBI Accession: NP_742152

UniProt: Q8IU54