DKK1 ELISA Kit

Catalog No. ABIN6720225

Product Details DKK1 ELISA Kit

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Gene Name: DKK1

Production: Oligomeric forms of natural and recombinant human DKK1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: T32-H266

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DKK1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK-1 (DKK1 Products)

Synonyms: DKK-1 ELISA Kit, SK ELISA Kit, AI414495 ELISA Kit, SGY ELISA Kit, SGY-1 ELISA Kit, SGY1 ELISA Kit, Soggy ELISA Kit, mSgy ELISA Kit, dkk1 ELISA Kit, dkk ELISA Kit, dkk-1 ELISA Kit, xdkk1 ELISA Kit, Xdkk-1 ELISA Kit, Dickkopf1 ELISA Kit, dickkopf-1 ELISA Kit, mdkk-1 ELISA Kit, dkk1-A ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 ELISA Kit, dickkopf like acrosomal protein 1 ELISA Kit, dickkopf-related protein 1 ELISA Kit, dickkopf-like 1 ELISA Kit, dickkopf WNT signaling pathway inhibitor 1b ELISA Kit, dickkopf protein ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 S homeolog ELISA Kit, DKK1 ELISA Kit, Dkk1 ELISA Kit, DKKL1 ELISA Kit, LOC100228789 ELISA Kit, Dkkl1 ELISA Kit, dkk1b ELISA Kit, dkk1 ELISA Kit, dickkopf ELISA Kit, dkk1.S ELISA Kit

Gene ID: 83430

NCBI Accession: NP_112542

UniProt: Q9EQ14

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation