Chitinase 3-Like 1/YKL-40 ELISA Kit
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- Target
- Chitinase 3-Like 1/YKL-40
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 62.5 pg/mL - 4000 pg/mL
- Minimum Detection Limit
- 62.5 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP-39, CGP39, CH3L1_HUMAN, CHI3L1, chitinase 3 like 1 (cartilage glycoprotein 39), chitinase 3 like 1, Chitinase 3 like protein 1 precursor, chitinase, Chitinase-3-like protein 1, GP 39, GP-39, GP39, HC gp39, HCGP 3P, hCGP-39, HCgp39, YKL 40, YKL-40, YKL40, YYL 40
Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40 kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Gene Name: CHI3L1
Production: Natural and recombinant human Chitinase 3-like 1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: Y22-T383
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Chitinase 3-Like 1/YKL-40 ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Chitinase 3-like 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each human Chitinase 3-like 1 standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 62.5pg/mL-4000pg/mL
Other Performance Data: DOE: 2-8° C - 8/23/18 -20° C - 2/23/19
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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