CCL21 ELISA Kit

Catalog No. ABIN6720217

Product Details CCL21 ELISA Kit

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 6Ckine, Beta chemokine exodus 2, C-C motif chemokine 21, Chemokine (C-C motif) ligand 21, CKb9, ECL, Efficient Chemoattractant for Lymphocytes, Exodus2, MGC34555, OTTHUMP00000000526, OTTHUMP00000000527, OTTHUMP00000021303, SCYA 21, SCYA21, Secondary lymphoid tissue chemokine, SLC, Small inducible cytokine A21, Small inducible cytokine subfamily A (Cys-Cys), member 21, TCA 4, TCA4

Background: Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Gene Name: CCL21

Production: Natural and recombinant human CCL21. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S24-P134

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL21 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: CCL21 - 6Ckine (CCL21 Products)

Synonyms: 6Ckine ELISA Kit, CKb9 ELISA Kit, ECL ELISA Kit, SCYA21 ELISA Kit, SLC ELISA Kit, TCA4 ELISA Kit, ccl21 ELISA Kit, Ccl21b ELISA Kit, C-C motif chemokine ligand 21 ELISA Kit, C-C motif chemokine 21 ELISA Kit, CCL21 ELISA Kit, ccl21 ELISA Kit, Ccl21 ELISA Kit

Gene ID: 6366

NCBI Accession: NP_002980

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization