Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

c-MET ELISA Kit

Rockland koa0436 MET Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720214
Supplier Product No.: koa0436
  • Target See all c-MET (MET) ELISA Kits
    c-MET (MET) (Met Proto-Oncogene (MET))
    Reactivity
    • 15
    • 10
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5 pg/mL - 4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0436
    Supplier
    Rockland
    Purpose
    Human C-MET - HGFR Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human C-MET. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 5 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product MET ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human C-MET standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: E25-R307(alpha)&S308-T932(beta)

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all c-MET (MET) ELISA Kits
    c-MET (MET) (Met Proto-Oncogene (MET))
    Alternative Name
    C-MET/HGFR (MET Products)
    Background

    Synonyms: AUTS9, c Met, Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene, Met proto oncogene tyrosine kinase, MET_HUMAN, Oncogene MET, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met

    Background: C-Met(MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor(HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

    Gene ID
    4233
    NCBI Accession
    NP_000236
    UniProt
    P08581
    Pathways
    RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
You are here:
Support