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MBL2 ELISA Kit

Rockland koa0476 MBL2 Reactivity: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720207
Supplier Product No.: koa0476
  • Target See all MBL2 ELISA Kits
    MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
    Reactivity
    • 8
    • 7
    • 6
    • 4
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0476
    Supplier
    Rockland
    Purpose
    Human MBP-C - MBL2 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human MBP-C. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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    Discover our top product MBL2 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MBP-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MBP-C standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: E21-I248

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all MBL2 ELISA Kits
    MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
    Alternative Name
    MBP-C/MBL2 (MBL2 Products)
    Background

    Synonyms: COLEC1, Collectin-1, Lectin mannose-binding soluble 2, Mannan binding lectin, Mannan binding protein, Mannose binding lectin 2 soluble, Mannose binding lectin 2 soluble, Mannose binding lectin, Mannose binding lectin protein C2 soluble opsonic defect, Mannose binding protein, Mannose binding protein C, Mannose binding protein C precursor, Mannose-binding lectin, MBL, MBL2_HUMAN, MBL2D, MBP 1, MBP, MBP1, MBPB, MBPC, MBPD, Opsonic defect, protein C, Soluble mannose binding lectin

    Background: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

    Gene ID
    4153
    NCBI Accession
    NP_000233
    UniProt
    P11226
    Pathways
    Complement System, Positive Regulation of Immune Effector Process
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