IL-20 ELISA Kit

Catalog No. ABIN6720199

Product Details IL-20 ELISA Kit

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Cytokine Zcyto10, Four alpha helix cytokine, IL-20, IL10D, IL20, IL20_HUMAN, Interleukin 20, Interleukin-20, MGC96907, UNQ852/PRO1801, ZCYTO10

Background: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Gene Name: IL20

Production: Natural and recombinant mouse IL-20. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: L25-E176

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-20 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL-20

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Alternative Name: IL-20 (IL20 Products)

Synonyms: IL-20 ELISA Kit, IL10D ELISA Kit, ZCYTO10 ELISA Kit, Zcyto10 ELISA Kit, IL20 ELISA Kit, interleukin 20 ELISA Kit, IL20 ELISA Kit, Il20 ELISA Kit

Gene ID: 58181

NCBI Accession: NP_001298020

UniProt: Q9JKV9

Pathways: Protein targeting to Nucleus