Decorin ELISA Kit

Catalog No. ABIN6720190

Supplier Product No.: koa0442

Product Details Decorin ELISA Kit

Target: Decorin (DCN)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Supplier Product No.: koa0442

Supplier: Rockland

Purpose: Mouse Decorin Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Decorin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Decorin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Decorin standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: D31-K354

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: Decorin (DCN Products)

Background:

Synonyms: Bone proteoglycan II, CSCD, DCN, DCN protein, Decorin, Decorin proteoglycan, Dermatan sulphate proteoglycans II, DKFZp686J19238, DSPG 2, DSPG2, PG 40 , PG II, PG S2, PG-S2, PG40, PGII, PGS 2, PGS2, PGS2_HUMAN, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B

Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size. It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Gene ID: 13179

NCBI Accession: NP_001177380

UniProt: P28654

Pathways: Glycosaminoglycan Metabolic Process