CD70 ELISA Kit

Catalog No. ABIN6720189

Supplier Product No.: koa0784

Product Details CD70 ELISA Kit

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Supplier Product No.: koa0784

Supplier: Rockland

Purpose: Mouse CD70 - TNFSF7 - cd27L Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CD70. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD70 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD70 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: S45-P195

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70/TNFSF7/cd27L (CD70 Products)

Background:

Synonyms: CD 27L, CD 70, CD27 L, CD27 LG, CD27 ligand, CD27-L, CD27L, CD27LG, CD70, CD70 antigen, CD70 Molecule, CD70_HUMAN, Ki 24 antigen, Ki24 antigen, Surface antigen CD70, TNFSF 7, TNFSF7, Tumor necrosis factor (ligand) superfamily member 7, Tumor necrosis factor ligand superfamily member

Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24 % amino acid sequence identity in the extracellular region.

Gene ID: 21948

NCBI Accession: NP_035747

UniProt: O55237

Pathways: Cancer Immune Checkpoints