CCL17 ELISA Kit

Catalog No. ABIN6720186

Product Details CCL17 ELISA Kit

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: A 152E5.3, ABCD 2, C C motif chemokine 17, C-C motif chemokine 17, CC chemokine TARC, CCL 17, CCL17, CCL17_HUMAN, Chemokine (C C motif) ligand 17, Chemokine ligand 17, MGC138271, MGC138273, SCYA17, Small inducible cytokine A17, Small inducible cytokine subfamily A (Cys Cys) member 17, Small inducible cytokine subfamily A member 17, Small-inducible cytokine A17, T cell directed CC chemokine, Thymus and activation regulated chemokine, Thymus and activation-regulated chemokine

Background: Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.

Gene Name: CCL17

Production: Natural and recombinant mouse CCL17. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A24-P93

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL17 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: CCL17 - TARC (CCL17 Products)

Synonyms: A-152E5.3 ELISA Kit, ABCD-2 ELISA Kit, SCYA17 ELISA Kit, TARC ELISA Kit, CCL17 ELISA Kit, Abcd-2 ELISA Kit, Scya17 ELISA Kit, Scya17l ELISA Kit, Tarc ELISA Kit, abcd2 ELISA Kit, C-C motif chemokine ligand 17 ELISA Kit, chemokine (C-C motif) ligand 17 ELISA Kit, ATP-binding cassette sub-family D member 2 ELISA Kit, CCL17 ELISA Kit, Ccl17 ELISA Kit, LOC100537809 ELISA Kit

Gene ID: 20295

NCBI Accession: NP_035462

UniProt: F6R5P4