IL18R1 ELISA Kit

Catalog No. ABIN6720173

Product Details IL18R1 ELISA Kit

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD218 antigen-like family member A, CD218a, CDw218a, CDw218a antigen, IL 1Rrp, IL-18R-1, IL-18R1, IL-1Rrp, IL1 receptor related protein, IL1 receptor-related protein, IL18R_HUMAN, IL18R1, IL18RA, Il18ralpha, IL1R-rp, IL1RRP, Interleukin 18 receptor 1, Interleukin 18 receptor alpha chain, Interleukin-18 receptor 1

Background: The interleukin-18 receptor 1(IL-18R1), also known as CDw218a(cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1(T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18(IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Gene Name: IL18R1

Production: Natural and recombinant human IL-18R1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: C22-R329

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-18R1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-18R1 standard solution and each sample be measured in duplicate.

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL18R1

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Alternative Name: IL-18R1 (IL18R1 Products)

Synonyms: CD218a ELISA Kit, CDw218a ELISA Kit, IL-1Rrp ELISA Kit, IL18RA ELISA Kit, IL1RRP ELISA Kit, IL-1R9 ELISA Kit, IL1R9 ELISA Kit, IL1RAPL-2 ELISA Kit, TIGIRR-1 ELISA Kit, Il18ralpha ELISA Kit, Il1rrp ELISA Kit, IL-18Ra ELISA Kit, IL18R1 ELISA Kit, interleukin 18 receptor 1 ELISA Kit, interleukin 1 receptor accessory protein like 2 ELISA Kit, IL18R1 ELISA Kit, IL1RAPL2 ELISA Kit, Il18r1 ELISA Kit

Gene ID: 8809

NCBI Accession: NP_001269328

UniProt: Q13478