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GDF15 ELISA Kit

GDF15 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720167
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    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Reactivity
    • 11
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC 1, NAG-1, NRG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PROSTATE-DERIVED FACTOR, PTGFB

    Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

    Gene Name: GDF15

    Production: Natural and recombinant human GDF-15. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: CHO, Immunogen sequence: A197-I308

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all GDF15 ELISA Kits
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Alternative Name
    GDF-15 (GDF15 Products)
    Synonyms
    GDF15 ELISA Kit, GDF-15 ELISA Kit, MIC-1 ELISA Kit, MIC1 ELISA Kit, NAG-1 ELISA Kit, PDF ELISA Kit, PLAB ELISA Kit, PTGFB ELISA Kit, SBF ELISA Kit, growth differentiation factor 15 ELISA Kit, GDF15 ELISA Kit, Gdf15 ELISA Kit
    Gene ID
    9518
    NCBI Accession
    NP_004855
    UniProt
    Q99988
    Pathways
    SARS-CoV-2 Protein Interactome
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