CD73 ELISA Kit

Catalog No. ABIN6720156

Product Details CD73 ELISA Kit

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 5' NT, 5' nucleotidase (CD73)|5' nucleotidase precursor, 5' nucleotidase ecto, 5' nucleotidase ecto (CD73), 5'-NT, 5'-nucleotidase, 5NTD_HUMAN, CD73, CD73 antigen, E5NT, Ecto 5' nucleotidase, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase

Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Gene Name: NT5E

Production: Natural and recombinant human CD73. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: W27-K547

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD73 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CD73 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73 - NT5E (NT5E Products)

Synonyms: CALJA ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, NTE ELISA Kit, eN ELISA Kit, eNT ELISA Kit, zgc:63784 ELISA Kit, NT5E ELISA Kit, MGC114869 ELISA Kit, PSPTO2277 ELISA Kit, 2210401F01Rik ELISA Kit, AI447961 ELISA Kit, Nt5 ELISA Kit, 5'-nucleotidase ecto ELISA Kit, 5'-nucleotidase, ecto (CD73) ELISA Kit, 5'-nucleotidase ELISA Kit, 5' nucleotidase, ecto ELISA Kit, NT5E ELISA Kit, nt5e ELISA Kit, nt5e.S ELISA Kit, PSPTO_2277 ELISA Kit, CBU_1233 ELISA Kit, MCA1322 ELISA Kit, Nt5e ELISA Kit

Gene ID: 4907

NCBI Accession: NP_001191742

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process