CD200 ELISA Kit

Catalog No. ABIN6720155

Product Details CD200 ELISA Kit

Target: CD200

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: antigen identified by monoclonal antibody MRC OX-2, CD_antigen=CD200, CD200 antigen, MOX1, MOX2, MRC OX-2 antigen, My033, OX-2 membrane glycoprotein

Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Gene Name: CD200

Production: Natural and recombinant human CD200. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q31-G232

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD200 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CD200 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD200

Target: CD200

Alternative Name: CD200 (CD200 Products)

Synonyms: MOX1 ELISA Kit, MOX2 ELISA Kit, MRC ELISA Kit, OX-2 ELISA Kit, CD200 ELISA Kit, Cspmo2 ELISA Kit, MRCOX2 ELISA Kit, Mox2 ELISA Kit, DKFZp459C0716 ELISA Kit, OX2 ELISA Kit, CD200 molecule ELISA Kit, Cd200 molecule ELISA Kit, CD200 antigen ELISA Kit, CD200 ELISA Kit, Cd200 ELISA Kit

Gene ID: 4345

NCBI Accession: NP_001004196

UniProt: P41217