CCL27 ELISA Kit

Catalog No. ABIN6720153

Product Details CCL27 ELISA Kit

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: ALP, C-C motif chemokine 27, CC chemokine ILC, Ccl27, CCL27_HUMAN, CTACK, CTAK, Cutaneous T cell attracting chemokine, Cutaneous T-cell-attracting chemokine, ESkine, IL 11 R alpha locus chemokine, IL 11 Ralpha locus chemokine, IL-11 R-alpha-locus chemokine, ILC, Ligand 27, PESKY, SCYA27, Skinkine, Small inducible cytokine A27, Small inducible cytokine subfamily A (Cys-Cys), member 27, Small-inducible cytokine A27

Background: Chemokine(C-C motif) ligand 27(CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine(ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine(CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.

Gene Name: CCL27

Production: Natural and recombinant human CCL27. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: F25-G112

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL27 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL27

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Alternative Name: CCL27 - CTACK (CCL27 Products)

Synonyms: ALP ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, CCL27 ELISA Kit, CTAK ELISA Kit, ESKINE ELISA Kit, PESKY ELISA Kit, SCYA27 ELISA Kit, AW558992 ELISA Kit, Ccl27 ELISA Kit, Scya27 ELISA Kit, Scya27a ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, uncharacterized LOC508933 ELISA Kit, chemokine (C-C motif) ligand 27A ELISA Kit, Ccl27 ELISA Kit, CCL27 ELISA Kit, LOC508933 ELISA Kit, Ccl27a ELISA Kit

Gene ID: 10850

NCBI Accession: NP_006655

UniProt: Q9Y4X3