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ESM1 ELISA Kit

Rockland koa0445 ESM1 Reactivity: Mouse Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720142
Supplier Product No.: koa0445
  • Target See all ESM1 ELISA Kits
    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    Reactivity
    • 9
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    23.4 pg/mL - 1500 pg/mL
    Minimum Detection Limit
    23.4 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0445
    Supplier
    Rockland
    Purpose
    Mouse ESM1 - Endocan Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant mouse ESM1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product ESM1 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: W20-R184

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all ESM1 ELISA Kits
    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    Alternative Name
    ESM1/Endocan (ESM1 Products)
    Background

    Synonyms: Endothelial cell-specific molecule 1, ESM-1, Esm1, ESM1_HUMAN

    Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).

    Gene ID
    71690
    NCBI Accession
    NP_076101
    UniProt
    Q9QYY7
    Pathways
    Growth Factor Binding
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