CD31 ELISA Kit
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- Target See all CD31 (PECAM1) ELISA Kits
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 156 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: CD31, CD31 antigen, EndoCAM, GPIIA, HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM 1, PECAM-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule
Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
Gene Name: PECAM1
Production: Natural and recombinant mouse PECAM-1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: E18-K590
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- Discover our top product PECAM1 ELISA Kit
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse PECAM-1 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all CD31 (PECAM1) ELISA Kits
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- PECAM-1 - CD31 (PECAM1 Products)
- Synonyms
- CD31 ELISA Kit, CD31/EndoCAM ELISA Kit, GPIIA' ELISA Kit, PECA1 ELISA Kit, PECAM-1 ELISA Kit, endoCAM ELISA Kit, C85791 ELISA Kit, Cd31 ELISA Kit, Pecam ELISA Kit, platelet/endothelial cell adhesion molecule 1 ELISA Kit, platelet and endothelial cell adhesion molecule 1 ELISA Kit, pecam1 ELISA Kit, PECAM1 ELISA Kit, Pecam1 ELISA Kit
- Gene ID
- 18613
- NCBI Accession
- NP_001027550
- UniProt
- Q08481
- Pathways
- Regulation of Actin Filament Polymerization
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