Leptin Receptor ELISA Kit

Catalog No. ABIN6720133

Product Details Leptin Receptor ELISA Kit

Target: Leptin Receptor (LEPR)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD295, HuB220, LEP-R, LEPR, LEPR_HUMAN, Leptin receptor, OB receptor, OB-R, OBR

Background: Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

Gene Name: LEPR

Production: Natural and recombinant mouse Leptin receptor. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence:  L22-G839

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: Leptin receptor (LEPR Products)

Synonyms: CD295 ELISA Kit, LEP-R ELISA Kit, LEPRD ELISA Kit, OB-R ELISA Kit, OBR ELISA Kit, obr ELISA Kit, cd295 ELISA Kit, ob-rb ELISA Kit, Fa ELISA Kit, LEPROT ELISA Kit, Leprb ELISA Kit, Modb1 ELISA Kit, OB-RGRP ELISA Kit, Obr ELISA Kit, db ELISA Kit, diabetes ELISA Kit, obese-like ELISA Kit, obl ELISA Kit, ObRb ELISA Kit, Ob-R ELISA Kit, Ob-RM ELISA Kit, lepr ELISA Kit, leptin receptor ELISA Kit, leptin receptor S homeolog ELISA Kit, LEPR ELISA Kit, lepr ELISA Kit, Lepr ELISA Kit, lepr.S ELISA Kit

Gene ID: 16847

NCBI Accession: NP_001116371

UniProt: P48356

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour