TPSAB1 ELISA Kit

Catalog No. ABIN6720119

Supplier Product No.: koa0539

Product Details TPSAB1 ELISA Kit

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Supplier Product No.: koa0539

Supplier: Rockland

Purpose: Human Tryptase - TPSAB1 B2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Tryptase. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 156pg/mL-10000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Tryptase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Tryptase standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: M1-V275

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: Tryptase/TPSAB1,B2 (TPSAB1 Products)

Background:

Synonyms: Tryptase-1, Tryptase1, Tryptase 1, TPS1, TPS2, TPSB1, Tryptase-2, Tryptase2, Tryptase 2, Tryptase-II, TryptaseII, Tryptase II, TPSB2, Tryptase alpha/beta-1, Tryptase beta-2

Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Gene ID: 7177

NCBI Accession: NP_003285

UniProt: Q15661