Galectin 3 ELISA Kit

Catalog No. ABIN6720103

Product Details Galectin 3 ELISA Kit

Target: Galectin 3 (LGALS3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin-3, GALIG, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LGALS2Mac-2 antigen, MAC2, Macrophage galactose-specific lectin

Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Gene Name: LGALS3

Production: Natural and recombinant human Galectin-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A2-I250

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3 - LGALS3 (LGALS3 Products)

Synonyms: CBP 35 ELISA Kit, Gal-3 ELISA Kit, Galectin-3 ELISA Kit, leg3 ELISA Kit, galactoside-binding ELISA Kit, galectin-3 ELISA Kit, lectin ELISA Kit, soluble ELISA Kit, CBP35 ELISA Kit, GAL3 ELISA Kit, GALBP ELISA Kit, GALIG ELISA Kit, L31 ELISA Kit, LGALS2 ELISA Kit, MAC2 ELISA Kit, MGC52947 ELISA Kit, MGC75803 ELISA Kit, LGALS3 ELISA Kit, gal-3 ELISA Kit, GBP ELISA Kit, L-34 ELISA Kit, Mac-2 ELISA Kit, gal3 ELISA Kit, galectin 3 ELISA Kit, Galectin-3 ELISA Kit, avian beta-defensin 3 ELISA Kit, lectin, galactoside-binding, soluble, 3 L homeolog ELISA Kit, lectin, galactoside-binding, soluble, 3 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose binding, soluble 3 ELISA Kit, LGALS3 ELISA Kit, leg3 ELISA Kit, AvBD3 ELISA Kit, lgals3.L ELISA Kit, lgals3 ELISA Kit, LGALS2 ELISA Kit, Lgals3 ELISA Kit

Target Type: Chemical

Gene ID: 3958

NCBI Accession: NP_002297

UniProt: P17931

Pathways: RTK Signaling