COMP ELISA Kit

Catalog No. ABIN6720100

Product Details COMP ELISA Kit

Target: COMP (Cartilage Oligomeric Matrix Protein (COMP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), Cartilage oligomeric matrix protein, Cartilage oligomeric matrix protein precursor, COMP, COMP_HUMAN, EDM 1, EDM1, EPD 1, EPD1, Epiphyseal dysplasia 1, Epiphyseal dysplasia 1 multiple, Epiphyseal dysplasia multiple 1, MED, MGC13181, MGC149768, PSACH, pseudoachondroplasia (epiphyseal dysplasia 1, multiple), Pseudoachondroplasia, THBS 5, THBS5, Thrombospondin 5, Thrombospondin-5, Thrombospondin5, TSP5

Background: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.

Gene Name: COMP

Production: Natural and recombinant human COMP. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-A757

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human COMP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human COMP standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Other Performance Data: DOE: 2-8° C - 12/14/18 -20° C - 06/14/19

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for COMP

Target: COMP (Cartilage Oligomeric Matrix Protein (COMP))

Alternative Name: COMP (COMP Products)

Synonyms: COMP ELISA Kit, EDM1 ELISA Kit, EPD1 ELISA Kit, MED ELISA Kit, PSACH ELISA Kit, THBS5 ELISA Kit, TSP5 ELISA Kit, cartilage oligomeric matrix protein ELISA Kit, COMP ELISA Kit, sce3551 ELISA Kit, CJA_1292 ELISA Kit, Comp ELISA Kit

Gene ID: 1311

NCBI Accession: NP_000086

UniProt: P49747