Cathepsin D ELISA Kit

Catalog No. ABIN6720097

Product Details Cathepsin D ELISA Kit

Target: Cathepsin D (CTSD)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CATD_HUMAN, Cathepsin D heavy chain, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, ctsd, Lysosomal aspartyl protease

Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Gene Name: CTSD

Production: Natural and recombinant human Cathepsin D. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L21-L412

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin D standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D (CTSD Products)

Synonyms: catD ELISA Kit, fb93e11 ELISA Kit, fj17b09 ELISA Kit, wu:fb93e11 ELISA Kit, wu:fj17b09 ELISA Kit, LOC398557 ELISA Kit, Cathd ELISA Kit, CatD ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, DKFZp469J0315 ELISA Kit, DDBDRAFT_0205785 ELISA Kit, DDBDRAFT_0215012 ELISA Kit, DDB_0205785 ELISA Kit, DDB_0215012 ELISA Kit, CLN10 ELISA Kit, CPSD ELISA Kit, CD ELISA Kit, cathepsin D ELISA Kit, cathepsin D S homeolog ELISA Kit, lysosomal aspartic protease ELISA Kit, cathepsin D-like ELISA Kit, ctsd ELISA Kit, LOC398557 ELISA Kit, ctsd.S ELISA Kit, CatD ELISA Kit, LOC5567565 ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, Cathd ELISA Kit, ctsD ELISA Kit, catd ELISA Kit, Ctsd ELISA Kit, LOC443060 ELISA Kit

Gene ID: 1509

NCBI Accession: NP_001900

UniProt: P07339

Pathways: Peptide Hormone Metabolism