Sialoadhesin/CD169 ELISA Kit

Catalog No. ABIN6720080

Product Details Sialoadhesin/CD169 ELISA Kit

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD169, CD169 antigen, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Sialic acid binding Ig like lectin 1, Sialic acid binding immunoglobulin like lectin 1, Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec 1, Siglec-1, Siglec1, SN, SN_HUMAN

Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

Gene Name: SIGLEC1

Production: Natural and recombinant mouse CD169. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T20-R1639

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD169 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD169 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169 - SIGLEC-1 (SIGLEC1 Products)

Synonyms: Cd169 ELISA Kit, Siglec-1 ELISA Kit, Sn ELISA Kit, CD169 ELISA Kit, SIGLEC-1 ELISA Kit, SN ELISA Kit, dJ1009E24.1 ELISA Kit, SA ELISA Kit, SIGLEC1 ELISA Kit, p210 ELISA Kit, pSn ELISA Kit, sialic acid binding Ig like lectin 1 ELISA Kit, sialic acid binding Ig-like lectin 1, sialoadhesin ELISA Kit, SIGLEC1 ELISA Kit, Siglec1 ELISA Kit

Gene ID: 20612

NCBI Accession: NP_035556

UniProt: Q62230