CXCL3 ELISA Kit

Catalog No. ABIN6720060

Supplier Product No.: koa0825

Product Details CXCL3 ELISA Kit

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Supplier Product No.: koa0825

Supplier: Rockland

Purpose: Mouse CXCL3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CXCL3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CXCL3 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: S32-S100

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for CXCL3 ELISA Kit (ABIN6720060)

Yang, Wu, Li, Wang, Mu, Hui, Li, Xu, Yang, Wei: "A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx." in: eLife, Vol. 11, (2022) (PubMed).

Target Details for CXCL3

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Alternative Name: CXCL3 (CXCL3 Products)

Background:

Synonyms: C-X-C motif chemokine 3, Chemokine (CXC motif) ligand 3, Cinc 2, Cinc2, CINC2b, CXCL3_HUMAN, Cytokine induced neutrophil chemoattractant 2, Dcip1, Dendritic cell inflammatory protein 1, Gm1960, GRO protein gamma, GRO-gamma, GRO-gamma(1-73), GRO-gamma(5-73), GRO3, GRO3 oncogene, GROG, Growth regulated protein gamma, Macrophage inflammatory protein 2 beta precursor, Melanoma growth stimulatory activity gamma, Member 3, MGSA gamma, MIP 2b, MIP2-beta, MIP2B, SCYB3, Small inducible cytokine subfamily B

Background: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Gene ID: 330122

NCBI Accession: NP_976065

UniProt: Q6W5C0

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy