IL-37 ELISA Kit

Catalog No. ABIN6720052

Product Details IL-37 ELISA Kit

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: FIL1, FIL1 zeta, FIL1(ZETA), FIL1Z, IL 1 zeta, IL 1F7, IL 1H4, IL 1RP1, IL 1X, IL 1X protein, IL-1 zeta, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-1X, IL-37, IL1F7, IL1F7_HUMAN, IL1H4, IL1RP1, Interleukin 1 family member 7, interleukin 1 family member 7 (zeta), Interleukin 1 homolog 4, Interleukin 1 related protein, Interleukin 1 superfamily z, Interleukin 1 zeta, Interleukin 37, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, Interleukin-23

Background: Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

Gene Name: IL37

Production: Natural and recombinant human IL-37. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: V46-D218

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-37 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-37 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL-37

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Alternative Name: IL-37 - IL1F7 (IL37 Products)

Synonyms: FIL1 ELISA Kit, FIL1(ZETA) ELISA Kit, FIL1Z ELISA Kit, IL-1F7 ELISA Kit, IL-1H ELISA Kit, IL-1H4 ELISA Kit, IL-1RP1 ELISA Kit, IL-37 ELISA Kit, IL1F7 ELISA Kit, IL1H4 ELISA Kit, IL1RP1 ELISA Kit, interleukin 37 ELISA Kit, IL37 ELISA Kit

Gene ID: 27178

NCBI Accession: NP_055254

UniProt: Q9NZH6