HBEGF ELISA Kit

Catalog No. ABIN6720047

Product Details HBEGF ELISA Kit

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Diphtheria toxin receptor, diphtheria toxin receptor (heparin-binding EGF-like growth factor), diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), DT R, DT-R, DTR, DTS, DTSF, HB-EGF, HBEGF, HBEGF_HUMAN, HEGFL, Heparin binding EGF like growth factor, Heparin binding epidermal growth factor, Heparin binding epidermal growth factor like growth factor, Heparin-binding EGF-like growth factor, Proheparin binding EGF like growth factor

Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

Gene Name: HBEGF

Production: Natural and recombinant human HBEGF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: D63-L148

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for HBEGF

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Alternative Name: HBEGF (HBEGF Products)

Synonyms: hbegf ELISA Kit, zgc:165443 ELISA Kit, HBEGF ELISA Kit, zgc:175091 ELISA Kit, DTR ELISA Kit, DTS ELISA Kit, DTSF ELISA Kit, HEGFL ELISA Kit, Dtr ELISA Kit, GFHB ELISA Kit, Hb-egf ELISA Kit, Hegfl ELISA Kit, AW047313 ELISA Kit, Dts ELISA Kit, HB-EGF ELISA Kit, heparin-binding EGF-like growth factor b ELISA Kit, heparin binding EGF like growth factor ELISA Kit, Heparin-binding EGF-like growth factor ELISA Kit, heparin-binding EGF-like growth factor a ELISA Kit, heparin-binding EGF-like growth factor ELISA Kit, hbegfb ELISA Kit, HBEGF ELISA Kit, hbegf ELISA Kit, hbegfa ELISA Kit, Hbegf ELISA Kit

Gene ID: 1839

NCBI Accession: NP_001936

UniProt: Q99075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway