CCL15 ELISA Kit

Catalog No. ABIN6720038

Supplier Product No.: koa0640

Product Details CCL15 ELISA Kit

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Supplier Product No.: koa0640

Supplier: Rockland

Purpose: Human CCL15 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CCL15. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL15 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: Q22-I113

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: CCL15 (CCL15 Products)

Background:

Synonyms: CC chemokine 3, CCL15, CCL15(29-92), CCL15_HUMAN, Chemokine CC-2, Chemokine CC2, HCC-2, HCC2, HMRP 2B, HMRP2B, Leukotactin 1, Leukotactin-1, LKN-1, LKN1, Macrophage inflammatory protein 5, MIP-1 delta, MIP-5, MIP1 delta, MIP1d, MIP5, Mrp-2b, Mrp2b, NCC-3, NCC3, SCYA15, SCYL3, Small inducible cytokine A15, Small inducible cytokine subfamily A (Cys-Cys), member 15, Small-inducible cytokine A15, SY15

Background: Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.

Gene ID: 6359

NCBI Accession: NP_116741

UniProt: Q16663