CCL1 ELISA Kit

Catalog No. ABIN6720036

Supplier Product No.: koa0343

Product Details CCL1 ELISA Kit

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Supplier Product No.: koa0343

Supplier: Rockland

Purpose: Human CCL1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CCL1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 4 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: K24-K96

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Alternative Name: CCL1 (CCL1 Products)

Background:

Synonyms: BF534419, C C motif chemokine 1, C-C motif chemokine 1, Ccl1, CCL1_HUMAN, CCR8 ligand, Chemokine CC Motif Ligand 1, Inflammatory cytokine I-309, P500, RP23-446K18.2, SCYA1, SISe, Small inducible cytokine A1, Small-inducible cytokine A1, T lymphocyte secreted protein I-309, T lymphocyte-secreted protein I-309, TCA3

Background: CCL1, Chemokine(C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Gene ID: 6346

NCBI Accession: NP_002972

UniProt: P22362