IL28A ELISA Kit
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- Target See all IL28A ELISA Kits
- IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 125 pg/mL - 8000 pg/mL
- Minimum Detection Limit
- 125 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0598
- Supplier
- Rockland
- Purpose
- Human IL-28A Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human IL-28A. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product IL28A ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human IL-28A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-28A standard solution and each sample be measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: V26-V200
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all IL28A ELISA Kits
- IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))
- Alternative Name
- IL-28A (IL28A Products)
- Background
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Synonyms: Cytokine ZCYTO20, EG330496, IFN-lambda-2, IFNL2, IL 28A, IL-28A, IL28A, IL28A_HUMAN, Interferon lambda 2, Interferon lambda-2, Interleukin 28A, Interleukin-28A
Background: Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B(IL28B), and interleukin 29(IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor(TLR)-induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.
- Gene ID
- 282616
- NCBI Accession
- NP_742150
- UniProt
- Q8IZJ0
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