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IL28A ELISA Kit

Rockland koa0598 IL28A Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720030
Supplier Product No.: koa0598
  • Target See all IL28A ELISA Kits
    IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))
    Reactivity
    • 12
    • 6
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125 pg/mL - 8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0598
    Supplier
    Rockland
    Purpose
    Human IL-28A Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human IL-28A. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product IL28A ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human IL-28A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-28A standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: V26-V200

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all IL28A ELISA Kits
    IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))
    Alternative Name
    IL-28A (IL28A Products)
    Background

    Synonyms: Cytokine ZCYTO20, EG330496, IFN-lambda-2, IFNL2, IL 28A, IL-28A, IL28A, IL28A_HUMAN, Interferon lambda 2, Interferon lambda-2, Interleukin 28A, Interleukin-28A

    Background: Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B(IL28B), and interleukin 29(IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor(TLR)-induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.

    Gene ID
    282616
    NCBI Accession
    NP_742150
    UniProt
    Q8IZJ0
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