IL17C ELISA Kit

Catalog No. ABIN6720029

Product Details IL17C ELISA Kit

Target: IL17C (Interleukin 17C (IL17C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CX2, Cytokine CX2, IL 17C, IL 21, IL-17C, IL17C, IL17C_HUMAN, Interleukin 17C, Interleukin-17C, MGC126884, MGC138401

Background: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Gene Name: IL17C

Production: Natural and recombinant human IL-17C. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: H19-V197

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human IL-17C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-17C standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 125pg/mL-8000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL17C

Target: IL17C (Interleukin 17C (IL17C))

Alternative Name: IL-17C (IL17C Products)

Synonyms: IL17C ELISA Kit, il17-3 ELISA Kit, CX2 ELISA Kit, IL-17C ELISA Kit, IL-21 ELISA Kit, interleukin 17C ELISA Kit, interleukin 17c ELISA Kit, IL17C ELISA Kit, il17c ELISA Kit, Il17c ELISA Kit

Gene ID: 27189

NCBI Accession: NP_037410

UniProt: Q9P0M4

Pathways: Cellular Response to Molecule of Bacterial Origin