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APCS ELISA Kit

Rockland koa0705 APCS Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720024
Supplier Product No.: koa0705
  • Target See all APCS ELISA Kits
    APCS (Amyloid P Component, Serum (APCS))
    Reactivity
    • 11
    • 8
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Supplier Product No.
    koa0705
    Supplier
    Rockland
    Purpose
    Mouse SAP - PTX2 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant mouse SAP. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 50 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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    Discover our top product APCS ELISA Kit
  • Application Notes

    ELISA_Dilution: 1.56 ng/mL-100 ng/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse SAP standard solution and each sample is measured in duplicate.

    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: Q21-D224

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all APCS ELISA Kits
    APCS (Amyloid P Component, Serum (APCS))
    Alternative Name
    SAP/PTX2 (APCS Products)
    Background

    Synonyms: 9.5S alpha-1-glycoprotein, APCS, SAMP_HUMAN, SAP, Serum amyloid P-component(1-203)

    Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterized by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

    Gene ID
    20219
    NCBI Accession
    NP_035448
    UniProt
    P12246
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