N-Cadherin ELISA Kit
-
- Target See all N-Cadherin (CDH2) ELISA Kits
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Supplier Product No.
- koa0384
- Supplier
- Rockland
- Purpose
- Human Cadherin-2 - N-Cadherin Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
-
Production: Natural and recombinant human Cadherin-2. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 20 pg/mL
- Components
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CDH2 ELISA Kit
-
-
- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Cadherin-2 standard solution and each sample is measured in duplicate.
- Comment
-
Standard: Expression system for standard: NSO, Immunogen sequence: D160-A724
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
-
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target See all N-Cadherin (CDH2) ELISA Kits
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- Alternative Name
- Cadherin-2/N-Cadherin (CDH2 Products)
- Background
-
Synonyms: CDw325, CDHN, Neural cadherin, N-cadherin, CD325
Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
- Gene ID
- 1000
- NCBI Accession
- NP_001295105
- UniProt
- P19022
- Pathways
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
-