CCL3 ELISA Kit

Catalog No. ABIN6720004

Product Details CCL3 ELISA Kit

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, Heparin binding chemotaxis protein, LD78-alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Gene Name: CCL3

Production: Natural and recombinant mouse CCL3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  A24-A92

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: CCL3 - MIP1 alpha (CCL3 Products)

Synonyms: MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit

Gene ID: 20302

NCBI Accession: NP_035467

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy