CCL4 ELISA Kit

Catalog No. ABIN6720003

Supplier Product No.: koa0259

Product Details CCL4 ELISA Kit

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Supplier Product No.: koa0259

Supplier: Rockland

Purpose: Human CCL4 - MIP-1 beta Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CCL4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human CCL4 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence:  A24-N92

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4/MIP-1 beta (CCL4 Products)

Background:

Synonyms: ACT 2, ACT2, AT744.1, C C motif chemokine 4, CCL4, CCL4L1, Chemokine CC Motif Ligand 4, G 26 T lymphocyte-secreted protein, HC21, LAG 1, LAG1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, MGC104418, MGC126025, MGC126026, MIP 1 beta, MIP1B, PAT 744, Protein H400, SCYA2, SCYA4, Secreted protein G 26, SIS gamma, Small inducible cytokine A4, T cell activation protein 2

Background: Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Gene ID: 388372

NCBI Accession: NP_002975

UniProt: P13236